Sample information

Order: Diptera

Family: Drosophilidae

Appearance: Small, tan, red eyes

Behavior: gather around ripe or rotting fruit

Defense: quickly fly away from danger

Ecological Role: help break down decaying fruit and recycle nutrients

Activity: active during the day

Methods

DNA Extraction

We dissected the Fruit Fly by isolating its abdomen to crush it for our sample DNA extraction.

Gel Electrophoresis

The following lane order for our gel:

1. N/A
2. N/A
3. Ladder
4. Sample #1 – Moth
5. Sample #2 – Rolly Polly
6. Sample #3 – Fly
7. Sample #4 – Pincher
8. Sample #5 – Spider
9. Wolbachia Positive Control
10. Wolbachia Negative Control
11. Positive DNA
12. N/A
13. N/A
14. N/A
15. N/A

Results

Given that the first run of our gel electrophoresis resulted in invalid data, we had to rerun our samples in our to get valid results. Our confidence levels were low at first but once we ran out gel and got bands for all of our wells. We are more than confident that our results are reliable due to our extensive precautions that we took and the multiple trial and errors.