GRP7 Armadillidium vulgare

May 22, 2026

Sample information
Picture
Entry by: Rae E., Chris R., Monica S., Jayda G., David C.
Location
Collection date 04/22/2026
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Caught in the bushes outdoors.
Putative identification Arthropoda Malacostraca Isopoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer 1X TAE
DNA stain UV Stain
Gel images
Protocol notes

We used a UV stain on a 1% agarose gel.

Well 1- Ladder

Well 2- Earbud

Well 3- Fly

Well 4- Centipede

Well 5- Positive Control for Wolbachia

Well 6- Negative Control for Wolbachia

Well 7- Positive DNA

Well 8- UV Stained Water

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Results for Wolbachia came back negative while DNA gel run was successful. Negative result indicates contamination was unlikely to occur and gel run showcases DNA was present.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Isopoda was found to be negative for Wolbachia.
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