GRP 10 Cosmopolitan Ground Beetle (Laemostenus complanatus)

May 22, 2026

Sample information
Picture
Entry by: Victor G.
Location
Collection date 04/27/2026
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Found under stones at White Point Beach
Putative identification Arthropoda Insecta Coleoptera Carabidae Laemostenus Laemostenus complanatus

Methods
Extraction kit QIAGEN DNeasy kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer 1X TAE
DNA stain 100X Fast Blast
Gel images
Protocol notes

1% agarose gel

165 volts 75 mAmps for 65 minutes

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
n/a
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
AGTAGGGACCTCTCTGAGAATACTAATTCGAGCAGAATTGGGTAACCCAGGAGCATTAATCGGTGATGATCAAATTTACAATGTTATTGTTACTGCCCATGCATTTGTAATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCCCCTGATATGGCCTTTCCACGAATAAACAATATAAGTTTTTGACTTCTTCCTCCATCTTTAACCCTCCTTTTAATAAGAAGAATAGTAGAAAGAGGGGCTGGAACTGGATGAACAGTCTATCCCCCTTTATCATCAGGAATTGCCCATAGAGGGGCTTCAGTTGATTTAGCAATTTTTAGATTACATTTAGCAAGAGTATCATCAATTCTTGGAGCTGTGAATTTTATTACTACAATTATCAATATACGATCAGTAGGAATAACTTTTGATCGAATGCCATTATTTGTTTGATCAGTTGGAATTACTGCTTTACTACTTCTCTTATCTTTGCCGGTATTAGCAGGAGCTATTACTATACTTCTTACAGATCGTAATCTTAATACATCCTTTTTTGACCCTGCAGGAGGGGGAGATCCAATTCTTTACCAACATTTATTCTGATTTTTTGGTCACCCTGGAAGTTTAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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