Sample information

Class: Arachnida

Order: Araneae 

Fang Orientation:

•  bite with a pinching, side-to-side motion (unlike tarantulas, which bite downwards).

Silk Usage

• Observe whether they use webs, bolas, or hunt freely. True spiders predominantly possess 6 spinnerets.

Respiration

• They rely on a mix of a single pair of book lungs and a tracheal (air tube) system for breathing.

Behavior:

• Males are typically smaller than females and often display elaborate, species-specific courtship vibrations or movements to avoid being eaten before mating

Methods

DNA Extraction

We dissected the Rolly Polly by isolating its abdomen to crush it for our sample DNA extraction.

Gel Electrophoresis

The following lane order for our gel:

1. N/A
2. N/A
3. Ladder
4. Sample #1 – Moth
5. Sample #2 – Rolly Polly
6. Sample #3 – Fly
7. Sample #4 – Pincher
8. Sample #5 – Spider
9. Wolbachia Positive Control
10. Wolbachia Negative Control
11. Positive DNA
12. N/A
13. N/A
14. N/A
15. N/A

Results

Given that the first run of our gel electrophoresis resulted in invalid data, we had to rerun our samples in our to get valid results. Our confidence levels were low at first but once we ran out gel and got bands for all of our wells. We are more than confident that our results are reliable due to our extensive precautions that we took and the multiple trial and errors.