Sample information

Found under rotting wood on rocks with same colony as Isopod-R #1 from on the rocks. It was at 8:00pm and it was 14°C outside.

Methods

DNA Extraction: This isopod was similar size to Isopod-R #1, its abdomen was also cut in half, and its exoskeleton scored with the scalpel. It was crushed well, but larger exoskeleton pieces remained and the liquid was discoloured, appearing pink.

Gel Electrophoresis – For CO1 arthropod

The first two wells got damaged so we had to move over:

3: NEB NO551L Ladder

4: Isopod #1

5: Isopod #2

6: Isopod-R #1

7: Isopod-R #2

8:

9:

10: Positive Control

11: Negative Control

12: DNA Postive Control

13: Water / DNA Negative Control

Wolbachia 16S

1. NEB NO551L Ladder

2. Isopod #1

3. Isopod #2

4.

5.

6. Isopod-R #1

7. Isopod-R#2

8.

9.

10. Positive Control

11. Negative Control

12. DNA Positive Control

13. Water / DNA Negative Control

Analysis: Our controls did not work and were likely contaminated, during Gel Electrophoresis no bands for both Arthropod CO1 or Wolbachia 16S appeared. However due to low confidence levels these results cannot be trusted.

Results

Our water (DNA – control) was contaminated with DNA, and our Wolbachia positive control did not indicate presence of Wolbachia. Therefore our confidence level is low and our results cannot be trusted.