Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/10/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | Yellowjacket wasp Slightly furry Thin wings |
| Putative identification | Arthropoda Insecta Hymenoptera Vespidae Vespula Vespula germanica |
Methods |
|
| Extraction kit | Qiagen Blood and Tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | This yellowjacket had some very bad smelling brown goop inside him instead of the normal clear one. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Honestly it doesn’t really seem like this insect had wolbachia from the gel electrophoresis but we had a few errors throughout the gel which is why you don’t see the positive drosophila control so it might be difficult to tell since one of the controls malfunctioned. Also we had a non-specific result. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
|
| Arthropod COI sequence |
|
| Summary | The Vespula germanica was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR