Long-Jawed Orchard Orbweaver

June 1, 2026

Sample information
Picture
Entry by: Mehran R.
Location
Collection date 04/22/2026
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

I discovered this spider on a set of dirt near a creek. The weather was sunny, and it was caught in the spring. The spider had a large white stripe on its back with a longer body. Its legs were green and longer.
Putative identification Arthropoda Arachnida Araneae Araneidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

I got a band for Wolbachia in my gels but it was fainter than the other Wolbachia positive band, so there may have been an error.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The controls worked as expected. I did not have any major problems with the protocol. I am confident that my insect had Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Araneidae was found to be postive for Wolbachia.
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