Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/14/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | The spider was found dangling on one of the trees in front of the school |
| Putative identification | Arthropoda Arachnida Opiliones |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | |
| Buffer | 1X TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | This sequence had amplified for COI but failed to amplify for Wolbachia. This leads us to conclude that |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Opiliones was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR