Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/15/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | Caught by Indian Run Stream. Winged arthropod that looks similar to a wasp. Moderate density of trees in a woodsy area. Arthropod was flying around a grassy, humid area. |
| Putative identification | Arthropoda Insecta Diptera Rhagionidae Chrysopilus Chrysopilus basilaris |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | We ran electrophoresis for both Cytochrome Oxidase from Drosophila(the top of the gel) and for Wolbachia-specific DNA from Drosophila(bottom of the gel) for four arthropod samples. Snipe Fly(Chrysopius basiloris) results are in the left most lane labeled Chrysopius basiloris. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Low confidence because the sample I focused on was the first Chrysopius basiloris sample which didn’t show a band for the Cytochrome Oxidase 1 which indicates that there might have been something wrong with the sample or other factors might have caused an issue. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR