Spider

May 29, 2026

Sample information
Picture
Entry by: Veda R
Location
Collection date 04/22/2026
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

This spider was caught by the stream near TJHSST. It was slightly drizzling at the time of capture, and it was found in a large pile of leaves and twigs near the stream at around 11:30 am.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Majority of our data did not result in visible bands which could be due to a lack of Wolbachia, or reagents that weren’t working properly. We can also see the negative control in the arthropod DNA showing up, which means something went wrong during the protocol.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Our arthropod DNA gel electrophoresis resulted in our negative control to show up, which isn’t supposed to happen, causing the rest of the results to be deemed invalid. More than that, no other sample had a clear band for the arthropod DNA. For the Wolbachia, the positive and negative controls did work properly, but due to the inconsistencies of the arthropod DNA, we are not too confident in our results.

 

For the fasta and ab1 upload, I’m not uploading anything because I didn’t get any viable DNA or Wolbachia levels from the gel electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
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