Male Clouded Sulphur Butterfly

May 29, 2026

Sample information
Picture
Entry by: Iryne J
Location
Collection date 04/15/2026
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

The arthropod was caught in a net while flying in an open field near flowers. The arthropod was caught on a warm and sunny afternoon. There were no other butterflies in the area, and the butterfly was flying in the field for 30 minutes before being caught.
Putative identification Arthropoda Insecta Lepidoptera Pieridae Colias Colias philodice

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

To collect the sample, I removed the abdomen from the thorax of the butterfly using a scalpel.

I did not need to dilute my sample, and it produced a 260/280 ratio of 1.67 with 73.839 ng/uL of nucleic acid.

My sample is the third sample from the left, and does not exhibit a Wolbachia band.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The Arthropod was visually identified as male and did not exhibit the phenotypical traits of a Wolbachia-positive Lepidoptera (feminization). Additionally, the gel results show single bands that do not cross the threshold, demonstrating a negative result for Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Colias philodice was found to be negative for Wolbachia.
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