Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/22/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations |
Sample was collected on a rock next to a stream. It was collected in the morning at around 11 AM and the temperature was 54 degrees F. |
| Putative identification | Arthropoda Insecta Hymenoptera Colletidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit. |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | I dissected the arthropod and ground the sample for more than 3 minutes. Analysis: My samples worked, but the negative insect control for CO1 showed a band. This indicates contamination, which may cause a false positive. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | The Wolbachia control showed a positive, which indicates that there likely wasn’t an error in the procedure that would cause a false negative. While one of our negative controls showed a band, this indicates a false positive could have happened. However, since our samples were negative, this error likely didn’t impact our Wolbachia gel. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Colletidae was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR