Snipe Fly

May 29, 2026

Sample information
Picture
Entry by: Shiv D.
Location
Collection date 04/15/2026
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

We found this near a grassy area with a moderate density of trees. I collected it in the morning around 11 AM, and the weather was sunny and around 85ยบ F. There were many snipe flies in the grassy area, but I only collected 1.
Putative identification Arthropoda Insecta Diptera Rhagionidae Chrysopilus Chrysopilus basilaris

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer 1X TBE
DNA stain SYBR Safe
Gel images
Protocol notes

DNA Extraction: I made sure to crush the arthropod really well and diluted my sample to meet the requirements for PCR.

We ran gel electrophoresis for both Cytochrome c Oxidase subunit from Drosophila (top of the gel) and for Wolbachia-specific DNA from Drosophila (bottom of the gel) for four arthropod samples. Chrysopius basiloris results are in the 4th lane for top and bottom gels labeled Chrysopius basiloris.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of my controls worked as expected, and I had no problems with the protocols. I wasn’t surprised by any of the bands on the gel; therefore, I am confident that the snipe fly wasn’t infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Chrysopilus basilaris was found to be negative for Wolbachia.
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