Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/29/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | Sample was collected on a rock next to a stream |
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | I ground the entire sample for over 5 minutes Analysis: My sample produced a band, but the negative insect control for CO1 showed a band. This indicates contamination, which may cause a false positive. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | The positive Wolbachia control produced the expected band, suggesting that there was likely no error that would have resulted in a false negative. Although one of the negative controls also showed a band, indicating that a false positive may have occurred, our samples remained negative. Therefore, this issue likely did not affect the interpretation of our Wolbachia gel results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR