Sample information

Found beneath bark of rotting tree stump on a sunny warm day at around 9:30 am.

Methods

DNA Extraction: I used a pestle and grains of sand to crush the arthropod. The entire insect was used as it was less than 2mm.

Sample: My sample 152 was the adult termite 2.

Gel Electrophoresis Lanes CO1/Wsp:

1- blank

2- termite 151

3- termite 152 (this specimen)

4- termite 153

5- blank

6- ladder

7- positive control Drosophila

8- negative control Drosophila

9- positive control DNA

10- water

11-13- blank

Analysis:

CO1: The bands for the termites were all the same length, which confirms that they are the same species. Furthermore, all of our three controls turned out as expected.

Wsp: None of our samples had clear bands, and only our positive control Drosophila did not work.

Results

Our confidence level is medium all of our negative controls worked, which prevent false positives. However, our positive control DNA for Wsp did not work, which means that it could possibly be a false negative, and this decreases our confidence. This means that our samples might potentially have Wolbachia but it did not show up in the gel.