Camponotus chromaiodes (Ferruginous carpenter ant, Wolbachia +)

June 4, 2026

Sample information
Picture
Entry by: Mia V.
Location
Collection date 05/29/2026
Captive / Cultivated? Wild-caught
Group Pingry School
Observations

Ferruginous Carpenter Ant found on the campus of the Pingry School, in a terrestrial habitat near a stream. The arthropod was caught in a sugar-soap trap.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Camponotus Camponotus chromaiodes

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

1% agarose gel made using 50mL 1X TAE buffer, 0.5g agarose powder, and 5μL Sybr Safe red. The gel was then loaded with a 1kg ladder, Wolbachia, and Arthopod DNA samples, and positive and negative DNA controls, and run at 100V.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

High confidence of Wolbachia positivity based on gel electrophoresis results and BLAST by the National Library of Medicine. Gel electrophoresis shows a Wolbachia fragment, signifying the presence of Wolbachia in the DNA sample.
Wolbachia 16S sequence Download FASTA   
GCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA   
TTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTGGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCCCTTATACTAGGATCTCCTGATATAGCTTACCCCCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGGACTGTCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGTATGGTCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGAGCTATTACAATACTACTAACAGACCGAAATCTTAATACTTCATTTTTCGACCCTTCGGGAGGGGGAGATCCTATTTTATACCAACATTTATT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Camponotus chromaiodes was found to be postive for Wolbachia.
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