Black Beetle #1

June 8, 2026

Sample information
Picture
Entry by: Anna S.
Location
Collection date 05/25/2026
Captive / Cultivated? Wild-caught
Group Winston Knoll Collegiate
Observations

We found this black beetle on a walking path in Lakeridge Park. We collected it at 11:50am, and it was a sunny day around 26° C. I attached a picture that I collected it from
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer 1X TBE
DNA stain SeeGreen
Gel images
Protocol notes

DNA extraction: We crushed the extracted abdomen of the beetle very well

Gel electrophoresis lanes for CO1 arthropod PCR:

1- DNA Ladder

2- Bug #1 Ladybug

3- Bug #2 Black beetle

4- Bug #3 Ladybug

5- Bug #4 Black beetle

6- + control

7- – control

8- + DNA control

9- Water

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of our controls worked as expected. We did not have any problems during the protocol, and we were not surprised by any bands on the gel. Therefore, we are confident that this arthropod is not infected with wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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