Sample information

This ladybug was found on the base of a tree near a walking path in Lakeridge Park. It was collected at 11:54am, it was caught on a sunny day, with a temperature of 26 degrees celsius. It was caught in the spring season on May 25th, 2026.

Methods

DNA extraction: The ladybug was throughly crushed before we began to dissect the abdomen out carefully, while making sure to not include the exoskeleton.

Gel electrophoresis lanes for CO1 arthropod PCR:

1- NEB NO551L ladder

2- Ladybug #1

3- Big Black Beetle #2

4-Ladybug #3

5- Small Black Beetle #4

6- +arthropod control

7- -Arthropod control

8- +DNA control

9- Water

Results

All of my controls worked as expected. I did not have any problems with the protocol, and ai was not surprised by any bands on the gel. Therefore, I am confident my arthropod is not infected with wolbachia.