Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 06/15/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Winston Knoll Collegiate |
| Observations |
|
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Lasius Lasius neoniger |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR's BlueGel |
| Buffer | 1X TBE |
| DNA stain | SeeGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Confidence level is medium because one of our two positive controls showed a band. Our negative control behaved as expected and did not show a band. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lasius neoniger was found to be negative for Wolbachia. |
Further away from water compared to ‘Wascana Wet’ samples. Drier environment. Also found in a patch of dirt in a field of grass. Grass is dry compared to the grass found around the ‘Wascana Wet’ samples. Less movement observed from the organism compared to the ‘Wascana Wet’ samples. Arthropod also did not want to surface and remained in the soil.
WKC – CAKA – Wascana Dryest Dry 2
WKC – CAKA – Wascana Dryest Dry 1
WKC – CAKA – Wascana Dry 2
WKC – CAKA – Wascana Dry 1
WKC – CAKA – Wascana Wet 2