WKC – Ariq C – Wascana Dry 1 – Lasius Neoniger

Sample information

Picture
Entry by: Ariq Chauhan
Location
Collection date 06/15/2026
Captive / Cultivated? Wild-caught
Group Winston Knoll Collegiate
Observations

Further away from water compared to ‘Wascana Wet’ samples. Drier environment. Also found in a patch of dirt in a field of grass. Grass is dry compared to the grass found around the ‘Wascana Wet’ samples. Less movement observed from the organism compared to the ‘Wascana Wet’ samples. Arthropod also did not want to surface and remained in the soil.

Putative identification Arthropoda Insecta Hymenoptera Formicidae Lasius Lasius neoniger

Methods

Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR's BlueGel
Buffer 1X TBE
DNA stain SeeGreen
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

Our confidence level is high because all of our controls worked as expected. In our arthropod gel, all of the wells showed a band except for the arthropod negative control, as expected. In our Wolbachia gel, our positive controls showed a band, whereas our negative controls did not, as expected. We are confident in the diagnosis of this arthropod.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lasius neoniger was found to be negative for Wolbachia.
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