Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 06/15/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Winston Knoll Collegiate |
| Observations |
|
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Lasius Lasius neoniger |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR's BlueGel |
| Buffer | 1X TBE |
| DNA stain | SeeGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | Confidence level is medium because one of our two positive controls showed a band. Our negative control behaved as expected and did not show a band. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lasius neoniger was found to be postive for Wolbachia. |
This arthropod was the furthest away from the water compared to the other samples, Wascana Wet and Wascana Dry. It was found on concrete tiles, not grass. In the environment this arthropod was found in, there were other ant species present, a phenomenon which was not observed in the other locations (Wascana Wet & Wascana Dry). There were also caterpillars present. We found this arthropod eating a dead caterpillar.
WKC – CAKA – Wascana Dryest Dry 2
WKC – CAKA – Wascana Dryest Dry 1
WKC – CAKA – Wascana Dry 2
WKC – CAKA – Wascana Dry 1
WKC – CAKA – Wascana Wet 2