WKC – Ariq C – Wascana Dryest Dry 2 – Lasius Neoniger

Sample information

Picture
Entry by: Ariq Chauhan
Location
Collection date 06/15/2026
Captive / Cultivated? Wild-caught
Group Winston Knoll Collegiate
Observations

This arthropod was the furthest away from the water compared to the other samples, Wascana Wet and Wascana Dry. It was found on concrete tiles, not grass. In the environment this arthropod was found in, there were other ant species present, a phenomenon which was not observed in the other locations (Wascana Wet & Wascana Dry). There were also caterpillars present. We found this arthropod eating a dead caterpillar.

Putative identification Arthropoda Insecta Hymenoptera Formicidae Lasius Lasius neoniger

Methods

Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR's BlueGel
Buffer 1X TBE
DNA stain SeeGreen
Gel images
Protocol notes

Results

Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Our confidence level is high because all of our controls worked as expected. In our arthropod gel, all of the wells showed a band except for the arthropod negative control, as expected. In our Wolbachia gel, our positive controls showed a band, whereas our negative controls did not, as expected. We are confident in the diagnosis of this arthropod.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lasius neoniger was found to be postive for Wolbachia.
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