Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 06/15/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Winston Knoll Collegiate |
| Observations |
|
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Lasius Lasius neoniger |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR's BlueGel |
| Buffer | 1X TBE |
| DNA stain | SeeGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Our confidence level is high because all of our controls worked as expected. In our arthropod gel, all of the wells showed a band except for the arthropod negative control, as expected. In our Wolbachia gel, our positive controls showed a band, whereas our negative controls did not, as expected. We are confident in the diagnosis of this arthropod. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lasius neoniger was found to be postive for Wolbachia. |

This arthropod was the furthest away from the water compared to the other samples, Wascana Wet and Wascana Dry. It was found on concrete tiles, not grass. In the environment this arthropod was found in, there were other ant species present, a phenomenon which was not observed in the other locations (Wascana Wet & Wascana Dry). There were also caterpillars present. We found this arthropod eating a dead caterpillar.
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR