Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 09/02/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | Red eyes, black or grey body, wings, furry on the body and legs. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | When making the PCR master mix, we thought we made enough for 10 samples, even though we had 9. However, we needed to make more because we ran out for some reason. Later on, we accidentally unplugged the PCR machine when we were trying to find an outlet for the gel electrophoresis machine. We started the PCR again, but it did not turn off when it was supposed to. When we tried to turn on the gel electrophoresis machine, it did not work at all. We had to wait a couple days to get another one and then it finally worked. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | There is no marker indicating that Wolbachia is present in the insect (03), but the line is a little blurred. The controls worked well, and the primer dimers showed up like they usually do. The controls worked on both the Wolbachia gel and the Arthropod gel. There was a band for the insect (03) in the arthropod gel. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diptera was found to be negative for Wolbachia. |
Water Boatman