Eastern Spotted-Winged Antlion

September 1, 2021

Sample information
Picture
Photos by: Solomon D., Sabina T., Sara P., and Sofia P.
Location
Collection date 08/21/2021
Captive / Cultivated? Wild-caught
Group The School for Science and Math at Vanderbilt
Observations

Caught while flying on a hot, sunny day.
Putative identification Arthropoda Hexapoda Insecta Neuroptera Myrmeleontidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

We were very exact with our procedure on the gel electrophoresis. Additionally, our controls were what was expected, including the positive and negative Wolbachia DNA controls, no band for the water, and the positive DNA control.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Myrmeleontidae was found to be negative for Wolbachia.
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