Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/21/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | The School for Science and Math at Vanderbilt |
| Observations | Caught while flying on a hot, sunny day. |
| Putative identification | Arthropoda Hexapoda Insecta Neuroptera Myrmeleontidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | We were very exact with our procedure on the gel electrophoresis. Additionally, our controls were what was expected, including the positive and negative Wolbachia DNA controls, no band for the water, and the positive DNA control. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Myrmeleontidae was found to be negative for Wolbachia. |
Water Boatman