DECMAVHemiptera

November 2, 2026

Sample information
Picture
Entry by: Madeline V
Location No map location data available.
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collected with sweep net in tall grass. Insect with orange body and black legs. The insect had a tube-like mouthpart.
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The gel electrophoresis showed a single line under the insect sample, meaning that the gel electrophoresis only detected insect DNA, and not Wolbachia DNA. My partner and I did not make mistakes on the gel electrophoresis. The controls appeared to show up how they were supposed to.
Wolbachia 16S sequence
Arthropod COI sequence
GTAGTAGGAACATCTTTAAGATGAATAATTCGTATTGAATTAGGAA CTCCTGGAACTTTTATTGGAAATGATCAAATTTATAATGTTTTCGTAACTGCACATGCATTTATTATAATTTTTTTTATA GTTATACCTATTATAATTGGAGGATTTGGTAATTGATTAGTGCCTTTAATAATTGGAGCTCCTGATATAGCCTTTCCACG AATAAATAATATAAGATTCTGACTCCTACCCCCATCTTTAACCTTGCTCTTAATTAGAAGAATTGCTGAAGGAGGTGCAG GAACAGGATGAACTGTGTACCCTCCTCTTTCTAGTAATATAGCTCATAGAGGAGCCGCTGTAGATTTAGCAATCTTCTCC TTACATTTAGCAGGGGTCTCCTCAATTCTAGGGGCAGTTAACTTTATCTCTACAATTATTAACATACGACCAGCCGGAAT AACTCCTGAACGAATTCCTTTATTCGTTTGGTCCGTAGGAATTACTGCCCTTCTTCTCCTTTTGTCATTACCTGTTCTAG CAGGTGCAATTACTATACTATTAACCGACCGAAATTTTAATACGTCATTTTTTGATCCATCGGGAGGAGGAGATCCTATT CTATACCAACACTTATTTTGATTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be negative for Wolbachia.
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