Protected: Hymenoptera

November 4, 2026

Sample information
Picture
Entry by: Kyle M
Location
Approximate location: 31.98, -81.16
Collection date
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Wings

  • Four-winged (2 pairs)
  • Membranous
  • Transparent
  • Clear
  • Uniform texture (same texture on both pairs)
  • Unequal (forewings larger than hindwings)
  • Few-celled (fewer than 20 wing cells)

Head

  • Mandibulate (chewing mandibles)
  • Large-eyed (prominent compound eyes)
  • Antennate (long, slender antennae)
  • Triangular

Body

  • Thin-waisted (narrow constriction between thorax and abdomen)
  • Segmented abdomen
  • Tapered/pointed abdomen
  • Elongated
  • Robust thorax

Classification

  • Hymenopteran
  • Terrestrial insect
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit container
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer lysis
DNA stain
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
TTGGAACATCCNTNNGATTAATTATTCGATTAGAACTAGGAA CTCCAATATCAATAATCAATAATGATCAAATTTACAACTCAATCATTACAGCACATGCATTAATTATAATTTTTTTTATA GTNNNNCCATTTATAATTGGGGGGTTCGGAAATTGATTAATTCCATTAATATTAGGAGCCCCAGATATAGCTTTCCCACG AATAAATAATATAAGATTCTGACTTCTACCACCATCTCTTATATTATTATTAATAAGAAATATTAATGATATAGGAGTAG GAACAGGATGAACTTTATATCCACCCCTATCATCAACTATAGGACACAATTCATCATCAGTAGATTTTGCTATTTTTTCA CTCCACATTGCAGGAATTTNNNNNNNCATAGGAGCNNNNNNNTTTATTGTAACCNNCNNAAATATACATATTAAAACCCA TTCATTAAATTTTTTACCTATATTTACATGATCAGTTTTAATCACAGCAATCTTATTACTATTATCTTTACCAGTTCTAG CAGGAGCNNNNACTANNCTATTAACTGACCGAAACTTAAATACATCATTTTTNTGNCCCTTTAGGAGGAGGAGANCCAAT TTTATTTCAACATTTANNNTGATTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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