Leafhopper Assassin Bug

October 6, 2021

Sample information
Picture
Photos by: Neena P.
Location No map location data available.
Collection date 08/22/2021
Captive / Cultivated? Wild-caught
Group The School for Science and Math at Vanderbilt
Observations

Found in the backyard in the evening when the porch light was left on.
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

There was a very faint bar for Wolbachia and no bar for arthropod, so one possible reason for this is that the primer was not compatible with this specific arthropod and that the arthropod may have been contaminated.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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