|Captive / Cultivated?
|The School for Science and Math at Vanderbilt
This white-fringed weevil was caught on the underside of a wooden mailbox. It was in the shade under a tree, in a neighborhood.
|Arthropoda Hexapoda Insecta Coleoptera Curculionidae
|DNA extraction location
|Single or Duplex PCR
|Gel electrophoresis system
*PCR machine failed during DNA amplification for 7 test tubes; the Bordenstein lab reran our samples.
*We identified this arthropod as a white-fringed weevil based on morphology and what BLASTN results suggested, but chromatogram results for CO1 were not completely clear, therefore our identification is not conclusive.
|Explanation of confidence level
High confidence level is due to appropriate (expected) test results from controls in both CO1 and WSPEC experiments. Positive controls were positive, negative control and water were Wolbachia negative, water was negative for arthropod DNA. There did not appear to be contamination between samples.
|Wolbachia 16S sequence
GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTGCCATCAGGTAATGCTGAGCACTTTAAGGAAACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCTTTATGGAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTAAAAAACATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTBLAST at The Wolbachia Project BLAST at NCBI
|Arthropod COI sequence
TTCGTTCAGAATTAGGAAACCCNGGATCTCTGTTGGAGATGACCAAATTTATAACGTTATTGTAACTGCTCATGCTTTTA TTATAATTTTCTTTATAGTAATACCTATCATAATTGGGGGATTTGGAAACTGACTAGTTCCATTAATACTAGGAGCTCCA GACATAGCCTTCCCTCGTCTTAATAATATAAGATTTTGACTACTTCCACCTTCTTTAACTTTACTTTTAATAAGAAGAAT TGTTGATAAAGGTGCAGGAACAGGATGAACAGTNTATCCCCCTCTCTCTGCCAATGTAGCTCATGAAGGATCCTCTGTTG ATTTAGCAATTTTTANACTACATATAGCAGGTGTATCATCAATTTTAGGAGCAGTAAATTTTATTTCTACAGTTTTAAAT ATACGACCTTCAGGAATATCTCCTGATCGAATACCTTTATTTGTATGAGCAGTAGAAATTACTGCTATNTTTATTACTTT TATCTTTACCAGTTTTAGCAGGAGCAATNACTATACTTTNTAACAGATCGAAATATTAATACCTCATTTTTTGACNCCTG CAGGAGGAGGAGATCCTATTTTATANCAACATTTATTTTGATTTTTTGGBLAST at The Wolbachia Project BLAST at NCBI
|The Curculionidae was found to be postive for Wolbachia.