Dinu’s Spider

November 1, 2021

Sample information
Picture
Photos by: Dinu D.
Location
Collection date 09/12/2021
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

The spider was caught inside a large web in the corner of a dark storage shed.
Putative identification Arthropoda Chelicerata Arachnida Araneae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The PCR reaction was ran twice to confirm the results.
Wolbachia 16S sequence Download FASTA    Download AB1
NNNNNNNNNNNNNNNNGGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TGGGGAGATTTATTGGAGATGATCATTTATATAATGTAATTGTTACGGCTCACGCTTTTGTTATGATTTTTTTTATAGTGATACCTATTTTAATTGGTGGGTTTGGAAATTGATTGGTTCCTTTAATATTAGGGGCTCCGGATATGGCTTTTCCTCGAATGAATAATTTGAGATTTTGATTGTTGCCTCCTTCGTTATTTATGCTTTTTATTTCTTCTATGGTGGATATAGGGGTTGGTGCTGGTTGAACAATTTATCCTCCTTTGGCTTCTTCTATGGGACATATAGGGAGAGCTATAGATTTTGCTATTTTTTCTTTGCATTTAGCAGGGGCGTCTTCTATTATGGGAGCGGTGAATTTTATTACTACGATTTTAAATATACGTTCTGTAGGGATAAGAATAGATAGGGTTTCTTTGTTTGTTTGGTCGGTATTGGTTACCGCTATTTTATTGTTATTGTCTTTGCCTGTATTAGCGGGTGCTATTACGATATTGTTGACGGATCGAAATTTTAATACATCATTTTTTGATCCTGCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Araneae was found to be postive for Wolbachia.
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