Agriphila ruricolella

November 3, 2021

Sample information
Picture
Photos by: Micah N.
Location
Collection date
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations
Putative identification Arthropoda Hexapoda Insecta Lepidoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

All our controls worked as expected and the results matched the gel electrophoresis.
Wolbachia 16S sequence Download FASTA    Download AB1
NNNNNNNNNNNNNNNNNGGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGC AACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGA TGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCG AGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGT AATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCN NTTTCNAAAAGCTA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
NNNTTTNNTTTTTGGATTTGAGCAGGAATAGTAGGAACATCCCTAAGATTACTAATTCGTGCTGAATTAGGAACCCCCGG ATCTTTAATTGGTGATGATCAAATTTATAATACTATTGTTACAGCTCATGCATTTATTATAATTTTTTTTATAGTAATAC CAATTATAATTGGTGGATTTGGAAATTGATTAGTTCCATTAATACTAGGAGCACCTGATATAGCATTCCCACGAATAAAT AATATAAGATTTTGATTATTGCCTCCCTCTTTAACCTTATTAATTTCTAGAAGAATTGTAGAAAATGGGGCTGGAACAGG ATGAACAGTATACCCCCCCCTTTCATCTAATATTGCTCACAGAGGAAGATCTGTAGACTTAGCTATTTTTTCCCTACACT TAGCAGGAATTTCTTCAATTCTAGGAGCAATTAATTTTATTACAACTATTATTAACATACGAATTAATGGATTATCTTTT GATCAAATACCTTTATTTGTTTGATCTGTTGGAATTACAGCTTTACTTCTTCTCCTATCATTACCAGTATTAGCTGGAGC TATTACTATATTACTTACTGATCGAAATCTTAATACTTCTTTTTTTGACCCTGCTGGAGGGGGTGACCCTATTTTATACC AACATTTATTTTGATTTTTGGNCNNNNNNNNNNNAAANNN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lepidoptera was found to be postive for Wolbachia.
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