Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/10/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Acton-Boxborough Regional High School |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | Our PCR test failed. All the DNA got leaked out of the well due to experimental error. Pipeheading was a fail after accidentally puncturing a hole at the bottom of the well. We got no results from our gel electrophoresis. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Vespidae Maculifrons
Tenebrio molitor
Wasp-Hymenoptera sp
Pollenia pediculata,
Phyciodes tharos