Sample information

Photos by: Matthew W.
Collection date 09/10/2021
Captive / Cultivated? Wild-caught
Group Acton-Boxborough Regional High School
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae


Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Well number four in the image above contains the Wolbachia DNA from our arthropod.


Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Our Wolbachia gel electrophoresis exhibits a defined band at a distance in correlation with the ladder, indicating Wolbachia presence. However, our control group for arthropod DNA extraction did not display a band, which makes us unsure about our DNA extraction. This lack of a band in our control may also be a result of faulty PCR for the Arthropod DNA.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Formicidae was found to be postive for Wolbachia.
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