Triplax

February 2, 2022

Sample information
Picture
Photos by: Divya T.
Location
Collection date 09/10/2021
Captive / Cultivated? Wild-caught
Group Acton-Boxborough Regional High School
Observations

This rove beetle was collected in the woods.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Erotylidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Because we also primed our collected DNA with arthropod primers and found that there were cut bands when we ran that gel, we know that we successfully extracted the DNA from the Triplax. This means that any issues with the Wolbachia band not showing up would not be chalked up to extraction errors, so we are fairly confident that the Triplax we found truly did not have Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Erotylidae was found to be negative for Wolbachia.
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