Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/23/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Singapore American School |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | PBS |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Formicidae was found to be postive for Wolbachia. |
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus
Fly- Pollen pediculata
Cricket- Acheta domesticus