PV’s Wolbachia Results

September 7, 2023

Sample information
Picture
Photos by: Paris V.
Location
Collection date 09/14/2021
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations
Putative identification Arthropoda Chelicerata Arachnida Araneae Theridiidae Steatoda Steatoda triangulosa

Methods
Extraction kit Edwards Buffer
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level
Wolbachia 16S sequence
Primers given by Wolbachia Project
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Primers given by Wolbachia Project
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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