Sample information
Picture	Photos by: Paris V.
Location
Collection date	09/14/2021
Captive / Cultivated?	Wild-caught
Group	Woburn Memorial High School
Observations
Putative identification	Arthropoda Chelicerata Arachnida Araneae Theridiidae Steatoda Steatoda triangulosa
Methods
Extraction kit	Edwards Buffer
DNA extraction location	Partial abdomen
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	Edvotek Gel Electrophoresis
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	Unknown
Confidence level	Low
Explanation of confidence level
Wolbachia 16S sequence	Primers given by Wolbachia Project BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence	Primers given by Wolbachia Project BLAST at The Wolbachia Project   BLAST at NCBI
Summary