Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/14/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Woburn Memorial High School |
| Observations | Small, green, winged insect |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera Cicadellidae |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | The actual results from this experiment cannot be determined due to no bands visible in the control wells; wells three and five. If the controls had worked, the conclusion would be that the arthropod tested did not contain Wolbachia, due to only one 708bp band appearing in the sample lane. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman