House Fly Results

April 13, 2022

Sample information
Picture
Location
Collection date 03/07/2022
Captive / Cultivated? Wild-caught
Group River Valley Community College
Observations

Arthropod was inside of enclosed environment, was not outside in the elements.
Putative identification Arthropoda Hexapoda Insecta Diptera Muscidae Musca

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

Both of the controls did not work, and did not show the results they were supposed to. Due to this, there is no way we can tell if the arthropod was either positive or negative.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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