Johnson, Coccinella septempunctata

Sample information

Picture
Photos by: Sawyer J.
Location
Collection date 02/23/2022
Captive / Cultivated? Wild-caught
Group River Valley Community College
Observations

Faint color on the outer shell

Putative identification Arthropoda Hexapoda

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

All positive and negative result provided the expected result

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be negative for Wolbachia.
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