Four-spot sap beetle

April 18, 2022

Sample information
Picture
Photos by: Savanna B
Location
Collection date 03/02/2022
Captive / Cultivated? Wild-caught
Group Richmond High School
Observations
  • Beetle
  • 1/4 inch
  • Shiny black
  • 4 yellow-brown spots
  • 6 legs
  • Found beneath wooden crate near rocks and several trees
Putative identification

Methods
Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

The addition of Buffer AW1 during DNA Purification cases the solution to turn pink.

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

The results are inconclusive because the DNA bands were not visible.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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