Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/30/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Richmond High School |
| Observations | Small body, black and brown body, hairy legs |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae |
Methods |
|
| Extraction kit | Thermoscientific GeneJet Genomic Purification |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | We tested our insects and they came out negative for Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Araneae was found to be negative for Wolbachia. |
Water Boatman