|Captive / Cultivated?||Wild-caught|
|Group||Richmond High School|
The roly-polys were together under a log, where it was cold and wet. The roly-polys liked to stay away from sunlight, mostly likely in dry and dark places in the dirt. I observed that it had 7 sets of legs, a hard plated dark grey exoskeleton, an oval body, and curled into a ball when touched, so I was able to identify that it was a pillbug.
|Putative identification||Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare|
|Extraction kit||Thermoscientific GeneJet Genomic Purification|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Duplex Reaction|
|Gel electrophoresis system||Edvotek Gel Electrophoresis|
|DNA stain||SYBR Safe|
I think that the DNA was not thoroughly cleaned and that enough was pulled out of the cells in the reproductive tissues of my arthropod.
|Explanation of confidence level||
Through gel electrophoresis, I saw that my pill bug tested inconclusive for Wolbachia because the RFLPS for my arthropod DNA did not show when we interpreted the gel under a fluorescent light. Because of the temperature and the way the gel was loaded with the small amount of DNA from my pillbug, I was unable to see if my arthropod was negative or positive for Wolbachia.
|Wolbachia 16S sequence||
|Arthropod COI sequence||