Wolbachia Project – RHS BI

Sample information

Photos by: Lydia C.
Collection date 03/30/2022
Captive / Cultivated? Wild-caught
Group Richmond High School

The roly-polys were together under a log, where it was cold and wet. The roly-polys liked to stay away from sunlight, mostly likely in dry and dark places in the dirt. I observed that it had 7 sets of legs, a hard plated dark grey exoskeleton, an oval body, and curled into a ball when touched, so I was able to identify that it was a pillbug.

Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare


Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

I think that the DNA was not thoroughly cleaned and that enough was pulled out of the cells in the reproductive tissues of my arthropod.


Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

Through gel electrophoresis, I saw that my pill bug tested inconclusive for Wolbachia because the RFLPS for my arthropod DNA did not show when we interpreted the gel under a fluorescent light. Because of the temperature and the way the gel was loaded with the small amount of DNA from my pillbug, I was unable to see if my arthropod was negative or positive for Wolbachia.

Wolbachia 16S sequence
Arthropod COI sequence
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