April 18, 2022

Sample information
Picture
Photos by: Cayden Lindahl
Location
Collection date 06/07/2022
Captive / Cultivated? Wild-caught
Group Richmond High School
Observations

It was a rolly Polley and it was under a rock
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

One person would make the gel and the other would do the pcr.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

Rolly Polleys were really hard to dissect
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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