Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 06/07/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Richmond High School |
| Observations | It was a rolly Polley and it was under a rock |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare |
Methods |
|
| Extraction kit | Thermoscientific GeneJet Genomic Purification |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | One person would make the gel and the other would do the pcr. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Rolly Polleys were really hard to dissect |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman