Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/31/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Richmond High School |
| Observations | |
| Putative identification | Arthropoda Myriapoda Chilopoda Scolopendromorpha |
Methods |
|
| Extraction kit | Thermoscientific GeneJet Genomic Purification |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | We switched wells 2 and 3 when filling them in |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | We did not have enough DNA to tell if the arthropod had wolbachia or not |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Vespidae Maculifrons
Tenebrio molitor
Wasp-Hymenoptera sp
Pollenia pediculata,
Phyciodes tharos