Pill Bug

Sample information

Photos by: Rachel S.
Collection date 03/30/2022
Captive / Cultivated? Wild-caught
Group Richmond High School

Walking under a log in wet soil. Curled into a small circle when poked.

Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae


Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

There was a lot of tubes passing around the table, which made it hard to determine whether or not I had used one, or even if I had only used it once or the recommended amount.


Wolbachia presence No
Confidence level Low
Explanation of confidence level

I am not confident that the arthropod did not have Wolbachia even when testing negative for it due to the main test results being inconclusive and unviable in reading.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidae was found to be negative for Wolbachia.
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