Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/11/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | three main parts of body with other ants in warm house six legs |
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Not extracting DNA right Contamination |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | Low because no arthropod band and positive control didn’t show it. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica