Ant

April 26, 2022

Sample information
Picture
Location
Collection date 04/11/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

three main parts of body

with other ants

in warm house

six legs
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Not extracting DNA right

Contamination

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Low because no arthropod band and positive control didn’t show it.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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