Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 04/04/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | When it was found the ant was walking around in circles on the ground with a quick pace. It was brown in color, had three body sections (a large lower section and a thin mid section), six legs (3 per side), 2 antennas, and two black eyes |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Enough DNA was extracted to have tested positive wolbachia band, but something must have gone wrong with the Arthropod PCR. This could have resulted due to the liquids being incorrect. The positive also didn’t result in an arthropod band. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | Even though it tested positive for wolbachia, the DNA didn’t result in a positive Arthropod test. Also the positive DNA control didn’t show as an arthropod. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be postive for Wolbachia. |
Water Boatman