Ant: CAL05

April 26, 2022

Sample information
Picture
Location
Collection date 04/04/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

When it was found the ant was walking around in circles  on the ground with a quick pace. It was brown in color, had three body sections (a large lower section and a thin mid section), six legs (3 per side), 2 antennas, and two black eyes
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Enough DNA was extracted to have tested positive wolbachia band, but something must have gone wrong with the Arthropod PCR. This could have resulted due to the liquids being incorrect. The positive also didn’t result in an arthropod band.

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

Even though it tested positive for wolbachia, the DNA didn’t result in a positive Arthropod test. Also the positive DNA control didn’t show as an arthropod.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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