Flat Backed Millipede

April 26, 2022

Sample information
Picture
Location
Collection date 04/12/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Around other aurthropods, moist environment, under rock, Long, round, many legs, brown, green part at end of tail,
Putative identification Arthropoda Myriapoda Diplopoda Polydesmida

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

A lot of people didn’t get results, so there may have been an error extracting the DNA.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

I collected an arthropod, but didn’t get an arthropod band. The positive control also did not show an arthropod band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Polydesmida was found to be negative for Wolbachia.
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