Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/12/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | Around other aurthropods, moist environment, under rock, Long, round, many legs, brown, green part at end of tail, |
| Putative identification | Arthropoda Diplopoda Polydesmida |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | A lot of people didn’t get results, so there may have been an error extracting the DNA. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | I collected an arthropod, but didn’t get an arthropod band. The positive control also did not show an arthropod band. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Polydesmida was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid