Flat Backed Millipede

Sample information

Collection date 04/12/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy

Around other aurthropods, moist environment, under rock, Long, round, many legs, brown, green part at end of tail,

Putative identification Arthropoda Myriapoda Diplopoda Polydesmida


Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

A lot of people didn’t get results, so there may have been an error extracting the DNA.


Wolbachia presence No
Confidence level Low
Explanation of confidence level

I collected an arthropod, but didn’t get an arthropod band. The positive control also did not show an arthropod band.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Polydesmida was found to be negative for Wolbachia.
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