April 27, 2022

Sample information
Picture
Photos by: Zack C
Location
Collection date 04/10/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations
  • Black body
  • Red legs (6)
  • Antennae
  • 1 set of wings
  • Size: pinky nail
Putative identification Arthropoda Hexapoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The DNA pellet at the bottom looked like a tan gunk glob.

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

Confidence level is very low because the negative control had Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be postive for Wolbachia.
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