Centipede

April 27, 2022

Sample information
Picture
Location
Collection date 04/12/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Terrestrial bug, has long legs, rough body, is in the ground
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

I collected an arthropod but didn’t get an arthropod band. The positive control didn’t have an arthropod band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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