Hyalella Azteca

April 28, 2022

Sample information
Picture
Photos by: Ella H.
Location
Collection date 04/10/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations
  • Very small
  • Shelled
  • Antennae
  • Crustacean
Putative identification Arthropoda Crustacea Malacostraca Amphipoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

It was difficult to put the mixture into the gel. I poked it a bit which worried me that the DNA would come out. Flipping the holder upside down for a long time gave me a similar worry. The pellet was light brown and all gathered at the bottom.

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level
  • I didn’t have an arthropod
  • Positive gel control didn’t show band for arthropod
  • Positive DNA showed for arthropod but had an odd double band
  • No band for Wolbachia DNA
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Amphipoda was found to be postive for Wolbachia.
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