Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 04/05/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | I noticed many observations of my arthropod during this lab. Such as its two wings that it used to fly around. How it had dark hair all over it body that matched is dark color. It big greenish eye that looked bigger then its body, those eyes could see 360 degrees. I also noticed how it was pretty harmless like many other bugs it had no claws or pincher or venom. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | Our first step was to collect the arthropod put it in a tube then killed it in rubbing alcohol. Then we picked up the dead bug with tweezer put in a Petri dish. Now that it was in the Petri dish I put under a microscope to examine it. Then transported back then we crushed up the bug then put it in a machine made it liquid, we did this a few more time then tested for wolbachia. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Because are positive band didn’t show wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diptera was found to be negative for Wolbachia. |
Water Boatman