Sample information

Collection date 04/05/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy

I noticed many observations of my arthropod during this lab. Such as its two wings that it used to fly around. How it had dark hair all over it body that matched is dark color. It big greenish eye that looked bigger then its body, those eyes could see 360 degrees. I also noticed how it was pretty harmless like many other bugs it had no claws or pincher or venom.

Putative identification Arthropoda Hexapoda Insecta Diptera


Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Our first step was to collect the arthropod put it in a tube then killed it in rubbing alcohol. Then we picked up the dead bug with tweezer put in a Petri dish. Now that it was in the Petri dish I put under a microscope to examine it. Then transported back then we crushed up the bug then put it in a machine made it liquid, we did this a few more time then tested for wolbachia.


Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Because are positive band didn’t show wolbachia.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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