Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 04/30/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Chrysomelidae Psylliodes Psylliodes cucullata |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | The line of BK07 shows no band of CO1, but the W-band alone is clearly visible. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
GAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTAT
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence |
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| Summary | The Psylliodes cucullata was found to be postive for Wolbachia. |
Harmonia Axyridis (Asian Lady Beetle)
(no title)
Brown Marmorated Stink Bug Wolbachia Project
Asian Lady Beetle Wolbachia Project
Melanoplus sp.